In vivo localization of chronically implanted electrodes and optic fibers in mice

2020. szeptember 17.

The most critical point in neurophysiology studies performed in experimental animals is the precise surgical targeting of brain areas. To minimize tissue damage, small electrodes or optic fibers, sometimes thinner than hair, are implanted. The small size of the mouse brain makes precise targeting extremely challenging even for expert operators. Researchers of the Institute of Experimental Medicine and Semmelweis University developed a method combining CT and MRI imaging, which enables precise implant localization directly after the surgery in live animals, in contrast to previously available histological methods. The method can increase the efficiency of mouse experiments, thus it has the potential to speed up neuroscience studies significantly. The reviewers also highlighted that, by sharing the CT-MRI data, the authors offered the possibility to other researchers to investigate e.g. individual variations in the morphology of brain areas. Bálint Király, Balázs Hangya, Krisztián Szigeti, Domokos Máthé and their colleagues presented the new procedure in the prestigious Nature Communications journal.

3 dimensional MRI image of the ventricles of the mouse brain


Bálint Király, Diána Balázsfi, Ildikó Horváth, Nicola Solari, Katalin Sviatkó, Katalin Lengyel,Eszter Birtalan, Magor Babos, Gergő Bagaméry, Domokos Máthé, Krisztián Szigeti & Balázs Hangya: In vivo localization of chronically implanted electrodes and optic fibers in mice.