3D functional network and dendritic imaging

Group 15
Leader: Balázs Rózsa

The focus of the group is the development of new 3D scanning methods for 3D two-photon microscopy, which allows scanning speed several order of magnitude faster than with previous solution even from awake, behaving animals. With the techniques published in Nature Methods and Neuron besides random access point scanning, we can measure multiple surface or volume element around the neurons on dendritic segment of interest without significant decrease in the scanning speed. Having the fluorescent information from these arbitrary surface elements and with the novel motion correction algorithms, we can retrieve the motion artifact free activity patterns from the regions of interest.

These results will help in the future to get better insight to neuronal assemblies. In the lab already multiple biological projects started using this technique. One of our major interests are the hippocampal activity patterns. The technique makes it possible to measure normal and abnormal functions of hippocampal oscillations in dendritic, cellular and population level. The contributions of ion channels in the physiological activity patterns was confirmed by pharmacological experiments.

In an other project we recorded the activity of pyramidal cell dendrites and different interneuron subtypes in vivo, in awake mice, with the help of two-photon microscopy. We investigated the representation of the reinforcement signals in the neocortex, and the effect of the cholinergic and serotonergic neuromodulatory inputs, while the mice performed an auditory discrimination task. With 3D-AO technique it is possible to measure in high number such a sparse interneuron population as the VIPs, which only make up 1% of the cortical cells. Our data shed light on how the reward and punishment influence the local microcircuits involved in sensory processing, and explore new details about the network model of contextual learning.

Excerpt from the Guidebook of the Institute 2015.

Group webpage: rozsalab.eu

Lab members:

Balázs RózsaGroup Leader
Gergely KatonaVisiting Scientist
Gergely SzalayAssistant Research Fellow
Linda JudákVisiting Researcher
Balázs ChioviniAssistant Research Fellow
Dénes PálfiVisiting Researcher
Zoltán SzadaiPhD Student
Tamás TompaVisiting Researcher
Miklós MadarászPhD Student
Gábor JuhászAssociated Research Fellow
Évi JámborTechnician
Alexandra BojdánTechnician
Domonkos PinkeUndergraduate Student
Lili BerényiUndergraduate Student
Blanka HeizerUndergraduate Student
Áron SzepesiAssociated Research Fellow
Máté MarosiVisiting Researcher
Csaba CsupernyákUndergraduate Student
Plauska AndriusAssociated Research Fellow