3DHISTECH Pannoramic MIDI II slide scanner S2
Wednesday, 20 July, 2022
Tags:
Microscopes
.
3DHISTECH Pannoramic MIDI II slide scanner
Main specification:
Camera:
PCO.edge 4.2 back illuminated sCMOS
Resolution: 2048x2048
Pixel size: 6.5 µm x 6.5 µm
Sensor format: 13.3 x 13.3 mm
Dynamic range: 16bit
Frame rate: 40 fps @ full resolution
Exposure: 2.0 ms the shortest exp. time in this system
Objectives:
Zeiss, Plan-Apochromat 10×/NA 0.3 M27 coverslip: #1.5
Zeiss, Plan-Apochromat 20×/NA 0.8 coverslip: #1.5
Zeiss, Plan-Apochromat 5×/NA 0.16 coverslip: #1.5 - ON DEMAND
Zeiss, Plan-Apochromat 40× Corr/NA 0.95 coverslip: #1.5 - ON DEMAND
Modalities:
Transmitted color brightfield (RGB illumination sequence)
EPI fluorescence (LED light source)
Pixel size:
10x (NA 0.45, resol:): 650nm,
20x (NA 0.8, resol:388nm): 325nm,
40x (NA 0.95, resol:327nm): ~162nm
Focusing:
One Tile (FOV):
10x objective: 2048*(6,5/10)=1331um, 1331umx1331um
20x objective: 2048*(6,5/20)=665um, 665umx665um
40x objective: 2048*(6,5/40)=332um, 332umx332um
Scan area:
approx: 21mm x 49.5mm (10x:~ 690 FOV, 20x: ~2700 FOV, 40x: ~11000 FOV)
Z range:320um (stepper motor step: 0.2um, range: 1050-2650)
Check your sample with the measuring tool, what can be found beside the scanner.
Light Source:
Lumencor, SPECTRA X light engine, LED
Fluorescent filters:
Quad band, DAPI, FITC, TRITC, CY5, CY3.5
Turning on-off:
|
Turning on: - Turn on the main switch on the extension cord Turning off: - Close the acquisition software Pannoramic Scanner 3.0.3 |
 |
Where to scan:
You have to scan to the following folder:
D:\Slides\your_group\your_name
When the scanning finished you have to MOVE your data to a network storage.
We clean up the D:\ drive regularly without any question
Manuals:
Pannoramic MIDI II:
Download Manual 3.0.2
Free viewer:
CaseViewer (view, export) Download Manual 2.3
Slide Converter Download Manual 2.3
Quant Center Download Manual 2.2
Scan Time, File size examples:
| Size | Objective | Channels | Filter | Z – Layers | Time | File Size |
| Full slide | 20x | 3 | 3 filters | 3 | 2.5 h | 9.7 Gb |
| 1/3 slide | 20x | 3 | 1 filter | 3 | 1.1 h | 8.2 Gb |
| Full slide | 20x | 1 | 1 filter | 1 | 18 min | 700 Mb |
Test image can be find (courtesy of Krisztina Horvath) \\kokifs2\kkk\other\3DHISTECH_Pannoramic_MidiII\Test_images/GFP_Cy3\4943_2.mrxs
you need the
\\kokifs2\kkk\other\3DHISTECH_Pannoramic_MidiII\Test_images\GFP_Cy3\4943_2 folder as well.