Nikon Fn1 A1R Multiphoton Microscope

Sunday, 20 March, 2022

Tags: Microscopes

The multiphoton confocal microscope enables excitation by simultaneous absorption of two or more near infrared photons by a single fluorescent molecule. Because of the small amount of absorption and scattering by the specimen, the near infrared light used for excitation penetrates deeper into the samples. An additional advantage is that the excited sample volume is diffraction limited in x-y-z, resulting in less photodamage and eliminates the need for a descanned pinhole. The resonant scanner is capable of imaging a wide area at 420 frames per second. The microscope has a large platform stage that can accommodate large samples - cells, tissues or whole organisms.

Overview

Component	Type	Description
Laser Lines	680 to 1080	Tunable Ti:Sapphire
Objectives	25x Water Dipping DIC*	CFI75 Apo LWD NA=1.1 WD=2mm FOV=?
Microscope Base	Nikon FN1 Eclipse	Upright
Stage Adapter	LN Bridge 500	Luigs&Neumann Slice Mini Bath Chamber
Acquisition Software	NIS Elements	Version:Version:4.3.00
Vibration Isolation Table	Supertech STable	Active pneumatic table
Widefield System
Diascopic illumination	FN-LH	Halogen lamp
Camera	Hamamatsu C7500-51 CCD Imaging Source DMK NME 41 AU02	NIR Interline CCD 560 × 575 pixels
Multiphoton System
Scanning Head	Nikon A1RMP+	High precision galvano and resonant scanners
Detector Unit:	A1-GNEF3	2 GaAsP NDD PMTs 2 Standard NDD PMT's
Emission Filters	550/88 nm 641/75 nm 732/68 nm	Nikon 492/SP Nikon 558/88 Nikon 641/75 Nikon 732/68

Schematic diagram of the lightpath

Details

Laser Lines:

Wavelength	Type	Manufacturer	Model	Power
680-1080nm Tunable	Ti:Sapphire	Coherent	Chameleon Vision II	1.5W at 710nm 3.0W at 800nm 1.35W at 920nm 400mW at 1040nm 180 mW at 1080nm

Part List:

Component	Type	ID	Description
Microscope Base	FN1 Eclipse Ti-PS100W/A Prior NIKRFK Prior Remote	S/N:842201 S/N:2116345 S/N:R021312273< S/N:R021312273/td>	Upright Base Controller Motorized Z focus Z focus remote
Nosepiece	Mad City Labs Nano-F200-N Mad City Labs ND1USB161	S/N:MCLS02978 S/N:MCLC02978	1 objective piezo Piezo Controller
Lasers
Tunable Laser	Chameleon Vision II	S/N:GDP.1001H.8613	Ti:Sapphire -
Pumping Laser	Coherent Verdi V Series 18V	S/N:SDP.1112171.11527	CW, 18W, 532nm
Accessories	Coherent T255P 1CR Coherent MRU X1	P/N:1234080 S/N:A43464 S/N:PPG.1133533.10494	Chiller Air Compressor Unit
Multiphoton System
Scanning Head	A1-SHRM A1-SHURM	S/N:31308 S/N:31308	Galvano scanners Controller
Detector Unit	A1-GNEF3 A1-NC	S/N:59504 S/N:550034	2 Standard/2 GaAsP NDD PMT-s S/N:92033
Laser Incident Unit	A1-IOU3	S/N:52007	Incident unit
Shutter	MP-ES	S/N:53036	Shutter
Widefield System
Diascopic Illumination	FN-LH FN1-DP	S/N:857169 - -	100W Halogen Lamp Double Port
Episcopic Illumination	MP-FN-E Intensilight C-HGFI	- S/N:671401	EpiFluor Adapter Enhanced Metal Arc (EmArc) Lamp
Camera	C7500-51 C2741-90	S/N:070085 S/N:540345	Hamamatsu CCD Hamamatsu Camera Controller
Micromanipulator Stage
Shifting Stage	LN V380FM-U	S/N:26C100124	Shifting Table (2D)
Stage Adapter	LN Bridge 500	P/N:200-100 500 0200	with Slice Mini Bath Chamber
Right Micromanipulator	Mini 25 XR Mini 25 YR Mini 25 ZR	S/N:14A100640 S/N:14B100588 S/N:14C100633	Luigs&Neumann Junior 4LE-InVivo(3axis Mini 25)
X/Y Controllers	LN SM7SN	S/N:610-S1407	Luigs&Neumann Controllers
Remote Controllers	LN TAS 7	S/N:500-T1413	Luigs&Neumann Remote controllers
Electrophysiological Components
Right Electrodes	MD 700B Axon CV-7B	S/N:836952 S/N:-	Molecular Devices Analog Amplifier Axon Inst. Headstage Right
Digitizer	Digidata 1322A	S/N:85559	Axon Instruments
Stimulation Isolator	World Precision Instruments	P/N:53650/VM2 S/N:135595	-
Peristaltic Pump	Watson Marlow 400	S/N:K121980	-
Uninterruptable Power Supply
Central Unit	Kstar MP SRTII 3kVA	S/N:310016538646305900002	Capacity:3000VA/2400W Battery Type 9Ah/72Vdc Battery Units 12
SNMP Card	SNMP Card DM801	-	Network adapter card
Battery Pack	Kstar RTII-BR12009 Kstar RTII-BR12009	S/N:310310777658286300001 S/N:310310777658286300001	Battery Type 9Ah/72Vdc Battery Units 12