Nikon Ni C2 Confocal Microscope
The C2 plus microscope is Nikon’s “work horse” laser scanning microscope. The scanning unit is equipped on a fully motorized Nikon Eclipse (Ni-E) microscope body. Our C2 confocal system enables users to acquire up to four channel images (available laser lines are: 405nm, 488nm, 561nm and 640nm) using 3 highly sensitive photomultiplier (PMT) detectors. Besides the confocal mode users can use the microscope in widefield mode with the Nikon Intensilight illuminator, a Nikon DS-Fi3 5.9 MP color CMOS camera and DAPI, FITC and TRITC filter cubes. The extremely precise motorized stage along with the motorized objective turret and filter wheel allow users to devise advanced imaging schemes. The microscope is equipped with high quality plan fluor and plan apo objectives (4X, 10x,20x, 60x). This very robust microscope is very user friendly and suits for most biological applications.
Overview
| Component | Type | Description |
|---|---|---|
| Laser Lines | 405 nm 488 nm 561 nm 640 nm |
100 mW* 100 mW* 20 mW* 100 mW* |
| Objectives | 4x 10x 20x 60x Oil |
Plan Fluor NA=0.13 WD=17.2mm FOV=3215.36um Plan Fluor NA=0.3 WD=16mm FOV=1290.4um Plan Apo VC NA=0.75 WD=1mm FOV=645.12um Plan Apo VC NA=1.45 WD=0.13mm FOV=215.04um |
| Microscope | Nikon Ni-E Eclipse | Upright, fully motorized (stage, filterwheel, turret) |
| Stage Adapter | Nikon Ni-SA | Standard microscope slides (25×75 mm) |
| Acquisition Software | NIS Elements AR | Version: 4.51.01 (Build 1146) |
| Confocal System | ||
| Confocal Scanning Head | C2si | High precision galvano scanners |
| Confocal Detector Unit: | C2-DU3 | 3 Standard PMTs |
| Emission Filters | DAPI/CY5(dual) 525/50 nm 595/40 nm |
Semrock EMD/CY5 Custom 239415 Nikon Nikon |
| Widefield System | ||
| Camera | Nikon DS-Fi3 |
CMOS Sensor: 2880x2048 pixels (6.91 × 4.92 mm, 2.4 um pixel size) ; color/emulated mono; QE=68%; 15 fps (2880 × 2048); 30fps (2x2 average / 1440 × 1024) |
| Illumination | Nikon Intensilight | Enhanced Metal Vapor Lamp |
| Filter Cubes |
DAPI |
EX: 340-380 nm; DM:400 nm; EM:435-485 nm EX: 465-495 nm; DM: 505 nm; EM: 515-555 nm EX: 527-552 nm; DM: 565 nm; EM: 577-632 nm EX:620/60 nm, DM:660 nm EM:700/71 nm |
*Nominal output power at laser head
Details
Laser Lines:
The Nikon LU4A laser box contains four lasers as follows:
| Wavelength | Type | Manufacturer | Model | Power | ID |
|---|---|---|---|---|---|
| 405 nm | Solid State | Coherent | CUBE | 100 mW | P/N, S/N:C18072403 |
| 488 nm* | Argon Ion | Melles Griot | IMA101010B0S | 17.7 mW | 35-IMA-410-019, S/N:M5193 |
| 561 nm | Solid State | Coherent | SAPPHIRE 561-20 CW CDHR | 20 mW | P/N: 1170380, S/N: LDP.1170380.505233 |
| 640 nm | Solid State | Coherent | OBIS 640 | 100 mW | P/N:, S/N: S200720010 |
*Argon ion lasers have addition emission lines at 458, 477 and 514 nm
Part List:
| Component | Type | ID | Description |
|---|---|---|---|
| Microscope Base | Ni-E Eclipse | S/N:910033 | Inverted, fully motorized (stage, filter wheel, turret) |
| Microscope Controller | Ni-SA | S/N:5400060 | - |
| xy-Stage | Ni-S-E | S/N:980096 | Motorized |
| Stage Adapter | Ni-SA | - | Standard microscope slides (25×75 mm) |
| Stage Controller | Ti-S-CON | S/N:562510 | - |
| Laser Board: | LU4A | S/N:60598 | 4 laser lines |
| Laser Controllers: | 35-INA-840-019 Saphire LP USB CDRH |
P/N:300-003 S/N:M6063-2 P/N:1170412 S/N:LDP.1170412.C00431 |
Melles Griot Argon Ion Controller Coherent Sapphire 561nm |
| Confocal System | |||
| Scanning Head | C2si C2-SHS |
S/N:33089 S/N:33089 |
High precision galvano scanners Controller |
| Detector Unit: | C2-DU3 | S/N:14172 | 3 Standard PMTs |
| Widefield System | |||
| Camera | DS-5Mc | - | 5Mp color camera |
| Diascopic Illumination | Intensilight C-HGFI | S/N:952280 | 30W Halogen Lamp |
| Episcopic Illumination | Intensilight C-HGFI | S/N:672778 | Enhanced Metal Arc (EmArc) Lamp Liquid lightguide |
| Fluorescent Attachment | Ni-FLEI EPI | P/N:MBE94100 | Manual ND filters |
| Filter Cube Turret | Ni-FLt6-E | P/N:MBE94300 | Motorized |
| Condensor | Ni-CUD-E | P/N:MBE99800 | Motorized |