Nikon STORM2 Microscope
Sunday, 20 March, 2022
Tags:
Microscopes
.
Starting the STORM2 system
Overview
| Component | Type | Description |
|---|---|---|
| Laser Lines | 405 nm 488 nm 561 nm 647 nm |
67 mW* 120 mW* 120 mW* 400 mW* Currently unavailable! |
| Objectives |
10x |
Plan Fluor NA=0.3 WD=16.00mm FOV=3215.36um |
| Microscope Base | Nikon Eclipse Ti2 | Inverted, fully motorized (stage, filterwheel, turret) |
| Stage Adapter | Ti-SH-U | Universal Holder (any microscope slide,35 mm dishes) |
| Acquisition Software | NIS Elements AR | Version: 5.21.00 |
| Vibration Isolation Table | Supertech STable | Active pneumatic table |
| DIC | ||
| Diascopic Illumination | Objective /DIC-slider | 20x CFI Plan Apo VC NA=0.75 / 20x 100x CFI SR HP Apochromat TIRF AC Oil / 100xI-R |
| Camera | HAMAMATSU C13440-20CU ORCA-Flash4.0 V3 |
sCMOS Camera 2048 (H)×2048 (V) pix. 6.5 μm×6.5 μm pixel size 13.312 mm×13.312 mm area 82 % (peak QE: @560 nm) |
| Epifluorescence System | ||
| Episcopic Illumination | CoolLED pE-4000 | Excitation Lines Recommended wavelengths/filter cubes and Possible wavelength combinations |
| Camera | HAMAMATSU C13440-20CU ORCA-Flash4.0 V3 |
sCMOS Camera 2048 (H)×2048 (V) pix. 6.5 μm×6.5 μm pixel size 13.312 mm×13.312 mm area 82 % (peak QE: @560 nm) |
| STORM/TIRF System | ||
| STORM/TIRF Illuminator | TI2-LA-NS2 | variable TIRF angle (2 motorized directions), 2x/4x/8x focusing lens |
| Filter Cubes |
DIC-analizator STORM CoolLED DAPI/FITC/TxRed** CoolLED CFP/YFP/mCherry** |
EX:387-417/483-494/557-570/636-661 DM:420-481/497-553/575-628/667-792 EM:422-478/502-549/581-625/674-786 EX:378/57_474/30_575/31 DM:414-450/499-546/604-850 EM:432/40_523/50_702/196 EX:475/27_543/27_702/201 DM:350-450/498-520/567-589 EM:438/29_509/26_578/25 |
| Camera | HAMAMATSU C13440-20CU ORCA-Flash4.0 V3 |
sCMOS Camera 2048 (H)×2048 (V) pix. 6.5 μm×6.5 μm pixel size 13.312 mm×13.312 mm area 82 % (peak QE: @560 nm) |
| Confocal System | ||
| Scanning Head | Nikon C2+ | High precision galvano scanners |
| Detector Unit: | C2-DU4 C2-DUT |
3 Standard PMTs 1 Diascopic PMT detector |
| 1st Dichroic Mirror | 405/488/561/640 | Nikon |
| Emission Filters | DAPI/CY5(dual) 427-463 nm/662LP 525/50 nm 594/40 nm |
Semrock D/CY5 Custom 239415 Nikon Nikon |
*Nominal output power at laser head
**Custom Filters with Chroma and/or semrock filters
Details
Laser Lines:
The Nikon LU4A laser box contains four lasers as follows:
| Wavelength | Type | Manufacturer | Model | Power |
|---|---|---|---|---|
| 405nm* | Solid State | Spectra-Physics | N1246-70021-CCI | 67mW |
| 488nm* | Solid State | Coherent | Sapphire 488-120 FP | 120mW |
| 561nm* | Solid State | Coherent | Sapphire 561-120 FP | 120mW |
| 647nm* | Solid State | MPB Communications Inc. | VFL-P-400-647-OEM2-B1 | 400mW |
*Nominal output power at laser head
*Laser power of STORM2 vs Ni-E C2
Part List:
| Component | Type | ID | Description |
|---|---|---|---|
| Microscope Base | Nikon Eclipse Ti2-E Ti2-CTRE Ti2-LA-CTR |
S/N:541848 S/N:451269 S/N:558145 |
Inverted, fully motorized Controller |
| xy-Stage/Focusing | Ti2-S-JS | S/N:128473 | Controller/Joystick/Focus |
| Stage Adapter | Ti-SH-U | - | Universal Holder (slides,35 mm dishes) |
| Piezo Stage | Mad City Labs Nano MCL nano Drive |
S/N:NIK-S1649 S/N:NIK-C1649 |
Piezoelectric Stage Controller |
| Nosepiece | - - |
- - |
Motorized nosepiece PFS Controller |
| Lasers | |||
|
Laser Board Laser Controller |
LU-NV-0 LASER UNIT LU-C |
S/N:60379 S/N:465253 |
4 laser lines Laser power supply |
| 405 Laser | Spectra-Physics N1246-70021-CCI | ||
| 488 Laser | Coherent Sapphire 488-120 FP | ||
| 561 Laser | Coherent Sapphire 561-120 FP | ||
| 647 Laser | MPB Communications Inc. VFL-P-400-647-OEM2-B1 | ||
| Confocal System | |||
| NATIONAL INSTRUMENTS PXI Chassis | PXI-1033 | S/N:3151D37 | * |
| Scanning Head | C2+ C2-SH |
S/N: S/N:432006 |
HP galvano scanners Controller |
| Detector Unit: | C2-DU3 | S/N:14656 | 3 Standard PMTs 1 Diascopic PMT detector |
| Widefield System | |||
| STORM/TIRF Illumination | TI2-LA-NS2 | S/N:592561 | Illumination Unit STORM Lens assembly |
| Diascopic Illumination | D-LH/LC Ti-DH Ti-CT-E |
S/N:955374 S/N:602303 - |
100W Halogen Lamp Illumination Pillar Manual/Intelligent Condensor |
| Episcopic Illumination | Intensilight C-HGFIE Controller - |
S/N:870068 S/N:620194 - |
1. Enhanced Metal Arc (EmArc) Lamp,Liquid lightguide 2. Fluorescent illumination unit 3. Motorized filter cube turret |
| Episcopic Illumination | CoolLED pE-4000 | S/N:CP0395 | Liquid lightguide |
| Camera | HAMAMATSU C13440-20CU ORCA-Flash4.0 V3 | S/N:301746 | sCMOS Camera 2048 (H)×2048 (V) pix. 6.5 μm×6.5 μm pixel size 13.312 mm×13.312 mm area 82 % (peak QE: @560 nm) |
| Cage Incubator/Temperature Unit/Touch screen interface | OKOLAB H201 T AIR HEATER/OKO-TOUCH | ?/S/N:032-1787/S/N:748-3282 | Gas type: 5%CO2 Temp. accuracy: ±0.3℃ at each heater surface Forced humidification by heating Bath Unit |
| Stage Top Incubator | TOKAI HIT/Model: STXF-TIZWX-SET | S/N:180470 | |
| Peristaltic Pump | WATSON MARLOW ALITEA Auto cased pump 50rpm | S/N:692607 | |