Nikon Ti A1R Confocal Microscope

Thursday, 29 September, 2022
Tags: Microscopes

The A1R was the high-end confocal solution from Nikon. Our system is equipped with 6 laser lines and 4 separate photomultiplier units and a spectral detector as well. The detector unit contains several dichroic mirrors and emission filters that can be changed using motorized filter wheels. The scanning head contains a hybrid scanner system incorporating both galvano and resonant scanners giving the user the extra option of high speed data acquisition. Using the resonant scanner one can scan a 515x512 pixel area at 30 frames per second. The two scanners can even be used simultaneously in photoactivation/photobleaching experiments. In these cases the galvano scanner is used for the activation of a given area and the resonant scanner is used for imaging. A separate PMT can be used to detect transmitted light. This can also be used to create DIC images with a polarizing DIC filter cube, a Nomarski prisms in the condenser/under the objective, and an addition polarizer. The scan head is mounted on a fully motorized Nikon Ti Eclipse inverted microscope with high quality plan fluor and plan apo objectives (4X, 10x,20x,40x,60x(Water), 60x(Oil)). Fluorescent episcopic illumination is achieved using the CoolLED pE-300 illumination system and a set of DAPI, FITC and TRIC filter cubes. The setup is well suited for live cell imaging as it is outfitted with a SOLENT environmental chamber with controlled temperature, humidity and CO2 atmosphere. This system is a very advanced tool with it's motorized components in combination with the JOBS module of the NIS Elements software package.

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Overview

 

Component Type Description
Laser Lines

405 nm

445 nm

488 nm

515 nm

561 nm

642 nm

50mW*

100mW*

60mW*

50mW*

20mW*

40mW*

Objectives 4x
10x
20x DIC**
40x
60x Water
60x Oil DIC**
Plan Fluor NA=0.13 WD=17.2mm FOV=3215.36um
Plan Fluor NA=0.3 WD=16mm FOV=1290.4um
Plan Apo VC NA=0.75 WD=1mm FOV=645.12um
S Plan Apo NA=0.6 WD=3.6-2.8mm FOV= ? um
Plan Apo VC NA=1.2 WD=0.31-0.28mm FOV=215.04um
Plan Apo VC NA=1.45 WD=0.13mm FOV=215.04um
Microscope Base Nikon Ti Eclipse Inverted, fully motorized (stage, filterwheel, turret)
Stage Adapter Ti-SH-U
Ti-SH-W
Ti-SH-J
Universal Holder (any microscope slide,35 mm dishes)
Well Plate Holder (24/96 well plates)
Stage Ring Holder (General purpose)
Acquisition Softwar NIS Elements AR Version: 5.20.02
Vibration Isolation Tables Supertech STable Active pneumatic table
Confocal System
Scanning Head Nikon A1R High precision galvano and resonant scanners
Detector Unit: A1-DU4
A1-DUS
A1-DUT
4 Standard PMTs
Spectral detector
Diascopic PMT detector
1st Dichroic Mirror 405/488
405/488/561
405/488/561/640
Beam Splitter 20/80
Nikon
Nikon
Nikon
Nikon
Nikon
Nikon
Emission Filters 425-75
500-550
572-622 nm
662-737
Nikon
Nikon
Nikon
Nikon
Widefield System
Illumination CoolLED pE-300  
Filter Cubes DAPI
FITC
TRITC
EX: 340-380 nm DM:400 nm EM:435-485 nm
EX: 465-495 nm DM: 505 nm EM: 515-555 nm
EX: 527-552 nm DM: 565 nm EM: 577-632 nm

*Nominal output power at laser head
**DIC Prism/Condensor: 20x (N2 cond./prism No.II) and 60x (N1 cond./prism No.I)

Details

Laser Lines:

The Nikon LU4A laser box and Omicron laser box(*) contain six lasers as follows:

Wavelength Type Manufacturer Model Power ID
408 nm Solid State Melles Griot 56RCS 50 mW P/N:S2780 S/N:00072073
18-Feb-2010
445 nm* Solid State Omicron LuxX-445-100 100 mW S/N:1906-120
488 nm* Solid State Omicron LuxX-488-60 60 mW S/N:1901-19
515 nm* Solis State Omicron LuxX-515-50 50 mW S/N:1901-20
561 nm DSSP Melles Griot 85-YCA-020-700 DSSP 20 mW

S/N:2173Y
Dec/2009

640 nm Solid State Coherent Cube 40 mW P/N:1170508/AC, S/N:C15120203
12/2/2015

 


Part List:

Component Type ID Description
Microscope Base Ti-E Eclipse
Ti-PS100W
Ti-HUBC/A
S/N:532257
S/N:2013094
S/N:551490
Inverted, fully motorized
Controller
Base Hub
xy-Stage Ti-S-ER
Ti-S-CON
Ti-S-EJOY
S/N:661168
S/N:561693
S/N:557697
Motorized with encoder
Controller
Joy-stick
Stage Adapter Ti-SH-U
Ti-SH-W
Ti-SH-J
-
-
-
Universal Holder (slides,35 mm dishes)
Well Plate Holder
Stage Ring Holder (General purpose)
Nosepiece Ti-ND6-PSF
Ti-PFS-CON
-
S/N:594808
Motorized nosepiece
PSF Controller
Laser Board LU4A S/N:60231 4 laser lines
Laser Controller LU-LR S/N:54488 Nikon Laser Controller
Confocal System
Scanning Head C2+
C2-SH
S/N:32045
S/N:32045
HP galvano scanners
Controller
Detector Unit: A1-DU4 S/N:55220 4 Standard PMTs
Widefield System
Diascopic Illumination D-LH/LC
Ti-PS100W
Ti-DH
Ti-CT-E
Ti-DIC
Ti-P2
S/N:955835
S/N:2013500
S/N:602697
-
-
-
100W Halogen Lamp
Lamp Controller
Diascopic Illumination Pillar
Motorized Condensor
DIC Lambda Plate
DIC Polarizer
Episcopic Illumination

CoolLED pE-300

S/N: CL2312

S/N: AN5916

Light Source

Control Pod

Filter Cube Turret Ti-FLC-E - Motorized
Incubation Chamber
Solent Scientific Ni-Ti-A1
T Controller
CO2 Controller
S/N:9761
S/N:9762
S/N:9763
Incubator chamber
T Controller
C02 Controller