in vitro

Sunday, 20 March, 2022
Tags: methods

Viral vector production and packaging

Viral constructs are "hybrid" molecules consisting of a recombinant carrier DNA molecule (vector) and the DNA to be expressed, i.e. an insert, which can be produced by molecular cloning in the quantities required for experiments. After amplification of the recombinant DNA containing the specific insert, the viral vectors, when introduced into the host cell, are able to efficiently infect the host cell and transfer the DNA to the host without causing an immune response.  Although the genes can be easily "transplanted" into other organisms, since the genetic code is universal throughout the living world, recombination prevents the viral vectors from replicating in the host organism.

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viral constructs